Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Alpha-mangostin attenuates oxidative stress and inflammation in adjuvant-induced arthritic rats

Yiwen Xu, Huimin Zhou, Lin Cai

Department of Orthopaedics, Zhongnan Hospital of Wuhan University, Wuhan, China;

For correspondence:- Lin Cai Email: lincai137@hotmail.com Tel:+8613476923997

Accepted: 12 October 2017 Published: 30 November 2017

Citation: Xu Y, Zhou H, Cai L. Alpha-mangostin attenuates oxidative stress and inflammation in adjuvant-induced arthritic rats. Trop J Pharm Res 2017; 16(11):2611-2616 doi: 10.4314/tjpr.v16i11.6

© 2017 The authors.
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Abstract

Purpose: To examine the protective effect of α-mangostin on Freund’s adjuvant-induced rheumatoid arthritis (RA) in rats.

Methods: Male Wistar rats were divided into four groups (n = 6). The rats were made arthritic using a single intradermal injection of 0.1 ml complete Freund's adjuvant. α-Mangostin (100 mg/kg, i.p.) was administered for 21 days. After the experimental period, the animals were sacrificed and various biochemical markers were assessed.

Results: Arthritis-induced rats showed a significant (p < 0.05) increase in malondialdehyde (16.28 ± 1.43 vs 7.22 ± 1.03) and nitric oxide (3.82 ± 0.87 vs 6.12 ± 1.23) with a concomitant reduction of antioxidants in the paw tissue. α-Mangostin intervention significantly (p < 0.05) reduced malondialdehyde and nitric oxide levels and restored antioxidant levels to normalcy by mitigating oxidative stress. The arthritic rats exhibited elevated tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) serum concentrations, upregulated TNF-x572; and IL–6 protein levels, and upregulated nuclear factor x581;B (NF-x581;B) mRNA expression in paw tissues. Treatment with α-mangostin significantly (p < 0.05) reduced serum levels and downregulated inflammatory markers to normalcy.

Conclusion: The results suggest that α-mangostin displayed a protective effect against adjuvant-induced arthritis in rats mediated through antioxidant and anti-inflammatory effects.

Keywords: Rheumatoid arthritis, Freundâ€™s adjuvant, ^5;-Mangostin, Oxidative stress, Inflammation